Study the levels of cytokines (IL-12 , IFN-γ) and human Proflin-1 (PFN-1)
Study the levels of cytokines (IL-12 , IFN-γ) and human Proflin-1 (PFN-1) in the sera of aborted women and healthy women (non pregnant and pregnant women)
Keywords:
Keywords: Toxoplasma gondii, cytokines (IL-12 , IFN-γ) and human proflin-1 (PFN-1)
Abstract
Abstract
Toxoplasma gondii an intracellular parasite infected large proportion of the world population, but mostly without visible clinical signs in immunocompetent patients. The present study was performed to determined the levels of cytokines (IL-12 , IFN-γ) and human proflin-1 (PFN-1) in the sera of 125 aborted women as case group and 30 healthy women (15 non pregnant and 15 pregnant women) as control groups in Women's and children Hospital in Al-Qadisiyah province from December / 2015 till March / 2016, Five ml of venous blood sample was obtained from each woman under sterile condition. Serum samples were positive for antibodies specific toxoplasmosis IgG and IgM. It was estimated levels of cytokines and profilin1 in these serums and significant increasing showed up in concentration interleukin 12 (IL-12) and profilin-1(PFN-1) and low in the concentration of interferon gamma (IFN-γ) at probability (0.05) in compare with control group that ensure their role in immune response against the parasite.
Keywords: Toxoplasma gondii, cytokines (IL-12 , IFN-γ) and human proflin-1 (PFN-1)
Downloads
Download data is not yet available.
References
References
1. Mckee, Amy S., et al. Functional inactivation of immature dendritic cells by the intracellular parasite Toxoplasma gondii. J Immunology, 2004, 173.4: 2632-2640.
2. Waree, Phuangphet. Toxoplasmosis: Pathogenesis and immune response.Thammasat Medical J, 2010, 8.4: 487-496.
3. AL-Azawi , B.M. and AL-Waeli , F.M.SStudies on the effect of infected cases of parasite Toxoplasma gondii on some sex hormones. J Pharmaceutical Research, 2015, 4.10: 2413-2423.
4. Radostits, O.M., Gay, C.C., Hinchcliff, K.W. and Constable, P.D. Veterinary Medicine: A textbook of the diseases of cattle, horses, sheep, pigs and goats.
Sounders, London, 2006, 10:1518-1522.
5. Kadir, Mohammed A., et al. Seroprevalence of Toxoplasma gondii among pregnant women in Kirkuk, Iraq. J Kir Univ-Sci Studies, 2011, 6: 1-11.
6. Kadhim, R. A., Al-marzoqi, A. H., & Al-taee, Z. M. Genetic susceptibility associated with toxoplasmosis ; genetic polymorphism , molecular and immunological study. J Al-Kufa University for Biology, 2016, 8.2: 21–38.
7. Mohammed, L. O., Ad’hiah, A. H., & Al-Nasiri, F. S. Cytokine gene polymorphisms of interleukin-1 receptor antagonist gene (IL1RNmspa11100) and its role in susceptibility or resistance to toxoplasmosis infection in samples of women from Salahaddin Governorate. International J of Current Microbiology and Applied Sciences, 2015, 4.9: 625–634. Retrieved from http://www.ijcmas.com/vol-4-9/Latif O. Mohammed, et al.pdf.
8. Abdul-Lateef, Huda Ibrahim. The Levels of IFN-gamma, IL-12 and Testosterone Hormone in Persons with Asymptomatic Toxoplasmosis, PhD Thesis. University of Baghdad, 2011.
9. Gazzinelli, Ricardo T., et al. Interleukin 12 is required for the T-lymphocyteindependent induction of interferon gamma by an intracellular parasite and induces resistance in T-cell-deficient hosts. Proceedings of the National Academy of Sciences, 1993, 90.13: 6115-6119.
10. E Sousa, Caetano Reis, et al. In vivo microbial stimulation induces rapid CD40 ligand–independent production of interleukin 12 by dendritic cells and their redistribution to T cell areas. J of experimental medicine, 1997, 186.11: 1819-1829.
11. Yap, George; PESIN, Michael; SHER, Alan. Cutting edge: IL-12 is required for the maintenance of IFN-γ production in T cells mediating chronic resistance to the intracellular pathogen, Toxoplasma gondii. J of Immunology, 2000, 165.2: 628-631.
12. Gazzinelli, Ricardo T., et al. Parasite-induced IL-12 stimulates early IFNgamma synthesis and resistance during acute infection with Toxoplasma gondii. J of Immunology, 1994, 153.6: 2533-2543.
13. Suzuki, Yasuhiro, et al. Interferon-gamma: the major mediator of resistance against Toxoplasma gondii. Science, 1988, 240.4851: 516.
14. Trinchieri, Giorgio. Interleukin-12 and the regulation of innate resistance and adaptive immunity. Nature Reviews Immunology, 2003, 3.2: 133-146.
15. Han, Seong-Ji. Visualization of immune cells during toxoplasma gondii infection. 2012. PhD Thesis. Freie Universität Berlin.
16. Evering, T.; Weiss, L. M. The immunology of parasite infections in immunocompromised hosts. Parasite immunology, 2006, 28.11: 549-565.
17. Sher, Alan; Denkers, Eric Y.; Gazzinelli, Ricardo T. Induction and regulation of host cell-mediated immunity by Toxoplasma gondii. In: Ciba Found Symp, 1995, 95-104.
18. Makhseed, M., et al. Th1 and Th2 cytokine profiles in recurrent aborters with successful pregnancy and with subsequent abortions. Human Reproduction, 2001, 16.10: 2219-2226.
19. Kareem, S. S. Prevalence, serodiagnosis and some immuno-logical aspects of toxoplasmosis among women in Baghdad province. 2008. PhD Thesis. M. Sc.
Thesis. College of health and Medical Technology. Technical Foundation.
104.
20. Fox, Barbara A.; Gigley, Jason P.; BZIK, David J. Toxoplasma gondii lacks the enzymes required for de novo arginine biosynthesis and arginine starvation triggers cyst formation. International j for parasitology, 2004, 34.3: 323-331.
21. Nascimento, Flávia RF, et al. Dual role for nitric oxide in paracoccidioidomycosis: essential for resistance, but overproduction associated with susceptibility. J of Immunology, 2002, 168.9: 4593-4600.
22. Rich, R. R.; Fleisher, T. A. ; Shearer, W. T.;Schroeder Jr., H. W.; Frew, A. J. and Weyand, C. M. Clincal immunology principles and practice , 2008, 3: 1578.
23. Andrade, Rosa M., et al. CD40 signaling in macrophages induces activity against an intracellular pathogen independently of gamma interferon and reactive nitrogen intermediates. Infection and immunity, 2005, 73.5: 31153123.
24. Ohshima, Jun, et al. RabGDIα is a negative regulator of interferon-γ–inducible
GTPase-dependent cell-autonomous immunity to Toxoplasma gondii.Proceedings of the National Academy of Sciences, 2015, 112.33: E4581-E4590.
25. Kim, Seon-Kyeong; Fouts, Ashley E.; Boothroyd, John C. Toxoplasma gondii dysregulates IFN-γ-inducible gene expression in human fibroblasts: insights from a genome-wide transcriptional profiling. J of Immunology, 2007, 178.8:
5154-5165.
26. Al-Moussawi, A. M., & Al-Qureshi, M. A. The study of cellular dynamics levels: interleukin β1-IL and interleukin IL-2 and interleukin IL-4 and interleukin IL-6 and interferon gamma IFN-γ in sero-positive women toxoplasmosis. J Babylon University Pure and Applied Sciences , 2015, 3 :23.
27. Al-Sorchee, S.M.A. Immunological study on women infected with Toxoplasmosis with a History of Abortion/Baghdad. M.Sc. Thesis. College of Education (Ibn Al- Haitham), University of Baghdad, 2005.
28. Abdullah D. A. ; Mahmood, M. A. ; Mohammed, D.B. The levels of cytokines
IL-4, IL-10, IL12P40, IFN-ˠ during acute Toxoplasmosis. J. Fac. Med. Baghdad, 2011, 53:4.
29. Abdul Mohymen, N. A., & Hussain. Detection of IL-10, IFN-γ and IL-8 in sera of patients with recurrent spontaneous abortion. Iraqi J of Medical Sciences, 2008, 6.2.
30. DI Nardo, Alessia, et al. Alternative splicing of the mouse profilin II gene generates functionally different profilin isoforms. J of cell science, 2000, 113.21: 3795-3803.
31. Bae, Yong Ho, et al. Profilin1 regulates PI (3, 4) P2 and lamellipodin accumulation at the leading edge thus influencing motility of MDA-MB-231 cells. Proceedings of the National Academy of Sciences, 2010, 107.50: 2154721552.
32. Shao, Jieya, et al. Phosphorylation of profilin by ROCK1 regulates polyglutamine aggregation. Molecular and cellular biology, 2008, 28.17:
5196-5208.
1. Mckee, Amy S., et al. Functional inactivation of immature dendritic cells by the intracellular parasite Toxoplasma gondii. J Immunology, 2004, 173.4: 2632-2640.
2. Waree, Phuangphet. Toxoplasmosis: Pathogenesis and immune response.Thammasat Medical J, 2010, 8.4: 487-496.
3. AL-Azawi , B.M. and AL-Waeli , F.M.SStudies on the effect of infected cases of parasite Toxoplasma gondii on some sex hormones. J Pharmaceutical Research, 2015, 4.10: 2413-2423.
4. Radostits, O.M., Gay, C.C., Hinchcliff, K.W. and Constable, P.D. Veterinary Medicine: A textbook of the diseases of cattle, horses, sheep, pigs and goats.
Sounders, London, 2006, 10:1518-1522.
5. Kadir, Mohammed A., et al. Seroprevalence of Toxoplasma gondii among pregnant women in Kirkuk, Iraq. J Kir Univ-Sci Studies, 2011, 6: 1-11.
6. Kadhim, R. A., Al-marzoqi, A. H., & Al-taee, Z. M. Genetic susceptibility associated with toxoplasmosis ; genetic polymorphism , molecular and immunological study. J Al-Kufa University for Biology, 2016, 8.2: 21–38.
7. Mohammed, L. O., Ad’hiah, A. H., & Al-Nasiri, F. S. Cytokine gene polymorphisms of interleukin-1 receptor antagonist gene (IL1RNmspa11100) and its role in susceptibility or resistance to toxoplasmosis infection in samples of women from Salahaddin Governorate. International J of Current Microbiology and Applied Sciences, 2015, 4.9: 625–634. Retrieved from http://www.ijcmas.com/vol-4-9/Latif O. Mohammed, et al.pdf.
8. Abdul-Lateef, Huda Ibrahim. The Levels of IFN-gamma, IL-12 and Testosterone Hormone in Persons with Asymptomatic Toxoplasmosis, PhD Thesis. University of Baghdad, 2011.
9. Gazzinelli, Ricardo T., et al. Interleukin 12 is required for the T-lymphocyteindependent induction of interferon gamma by an intracellular parasite and induces resistance in T-cell-deficient hosts. Proceedings of the National Academy of Sciences, 1993, 90.13: 6115-6119.
10. E Sousa, Caetano Reis, et al. In vivo microbial stimulation induces rapid CD40 ligand–independent production of interleukin 12 by dendritic cells and their redistribution to T cell areas. J of experimental medicine, 1997, 186.11: 1819-1829.
11. Yap, George; PESIN, Michael; SHER, Alan. Cutting edge: IL-12 is required for the maintenance of IFN-γ production in T cells mediating chronic resistance to the intracellular pathogen, Toxoplasma gondii. J of Immunology, 2000, 165.2: 628-631.
12. Gazzinelli, Ricardo T., et al. Parasite-induced IL-12 stimulates early IFNgamma synthesis and resistance during acute infection with Toxoplasma gondii. J of Immunology, 1994, 153.6: 2533-2543.
13. Suzuki, Yasuhiro, et al. Interferon-gamma: the major mediator of resistance against Toxoplasma gondii. Science, 1988, 240.4851: 516.
14. Trinchieri, Giorgio. Interleukin-12 and the regulation of innate resistance and adaptive immunity. Nature Reviews Immunology, 2003, 3.2: 133-146.
15. Han, Seong-Ji. Visualization of immune cells during toxoplasma gondii infection. 2012. PhD Thesis. Freie Universität Berlin.
16. Evering, T.; Weiss, L. M. The immunology of parasite infections in immunocompromised hosts. Parasite immunology, 2006, 28.11: 549-565.
17. Sher, Alan; Denkers, Eric Y.; Gazzinelli, Ricardo T. Induction and regulation of host cell-mediated immunity by Toxoplasma gondii. In: Ciba Found Symp, 1995, 95-104.
18. Makhseed, M., et al. Th1 and Th2 cytokine profiles in recurrent aborters with successful pregnancy and with subsequent abortions. Human Reproduction, 2001, 16.10: 2219-2226.
19. Kareem, S. S. Prevalence, serodiagnosis and some immuno-logical aspects of toxoplasmosis among women in Baghdad province. 2008. PhD Thesis. M. Sc.
Thesis. College of health and Medical Technology. Technical Foundation.
104.
20. Fox, Barbara A.; Gigley, Jason P.; BZIK, David J. Toxoplasma gondii lacks the enzymes required for de novo arginine biosynthesis and arginine starvation triggers cyst formation. International j for parasitology, 2004, 34.3: 323-331.
21. Nascimento, Flávia RF, et al. Dual role for nitric oxide in paracoccidioidomycosis: essential for resistance, but overproduction associated with susceptibility. J of Immunology, 2002, 168.9: 4593-4600.
22. Rich, R. R.; Fleisher, T. A. ; Shearer, W. T.;Schroeder Jr., H. W.; Frew, A. J. and Weyand, C. M. Clincal immunology principles and practice , 2008, 3: 1578.
23. Andrade, Rosa M., et al. CD40 signaling in macrophages induces activity against an intracellular pathogen independently of gamma interferon and reactive nitrogen intermediates. Infection and immunity, 2005, 73.5: 31153123.
24. Ohshima, Jun, et al. RabGDIα is a negative regulator of interferon-γ–inducible
GTPase-dependent cell-autonomous immunity to Toxoplasma gondii.Proceedings of the National Academy of Sciences, 2015, 112.33: E4581-E4590.
25. Kim, Seon-Kyeong; Fouts, Ashley E.; Boothroyd, John C. Toxoplasma gondii dysregulates IFN-γ-inducible gene expression in human fibroblasts: insights from a genome-wide transcriptional profiling. J of Immunology, 2007, 178.8:
5154-5165.
26. Al-Moussawi, A. M., & Al-Qureshi, M. A. The study of cellular dynamics levels: interleukin β1-IL and interleukin IL-2 and interleukin IL-4 and interleukin IL-6 and interferon gamma IFN-γ in sero-positive women toxoplasmosis. J Babylon University Pure and Applied Sciences , 2015, 3 :23.
27. Al-Sorchee, S.M.A. Immunological study on women infected with Toxoplasmosis with a History of Abortion/Baghdad. M.Sc. Thesis. College of Education (Ibn Al- Haitham), University of Baghdad, 2005.
28. Abdullah D. A. ; Mahmood, M. A. ; Mohammed, D.B. The levels of cytokines
IL-4, IL-10, IL12P40, IFN-ˠ during acute Toxoplasmosis. J. Fac. Med. Baghdad, 2011, 53:4.
29. Abdul Mohymen, N. A., & Hussain. Detection of IL-10, IFN-γ and IL-8 in sera of patients with recurrent spontaneous abortion. Iraqi J of Medical Sciences, 2008, 6.2.
30. DI Nardo, Alessia, et al. Alternative splicing of the mouse profilin II gene generates functionally different profilin isoforms. J of cell science, 2000, 113.21: 3795-3803.
31. Bae, Yong Ho, et al. Profilin1 regulates PI (3, 4) P2 and lamellipodin accumulation at the leading edge thus influencing motility of MDA-MB-231 cells. Proceedings of the National Academy of Sciences, 2010, 107.50: 2154721552.
32. Shao, Jieya, et al. Phosphorylation of profilin by ROCK1 regulates polyglutamine aggregation. Molecular and cellular biology, 2008, 28.17:
5196-5208.
Published
2021-07-19
How to Cite
Hadi, H. S., Raad A. Kadhim, & Raheem T. O. Al-Mammori. (2021). Study the levels of cytokines (IL-12 , IFN-γ) and human Proflin-1 (PFN-1). Al-Qadisiyah Journal of Pure Science, 26(4), 146–155. https://doi.org/10.29350/qjps.2021.26.4.1352
Section
Special Issue (Silver Jubilee)
Copyright © Author(s) . This is an open access article distributed under the Creative Commons Attribution
License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly
cited.
