Detection of Vibrio parahaemolyticus by polymerase chain reaction (PCR) technique

Abstract

Vibrio parahaemolyticus causes gastroenteritis in humans, this bacterium lives in coastal water during the summer, repetitive extragenic palindromic PCR (REP-PCR), one of the fingerprinting technique that used to analyse the isolates of V. parahaemolyticus tested in the current study, the majority of clinical isolates produced a band at 1000 bp amplicon. The DNA from this band was cloned and sequenced and found to code for a peptide chain release factor 1. The specific PCR amplification results showed that all 100% of the all V. parahaemolyticus strains tested were positive for 400 bp products. None of the other Vibrio species and non-Vibrio produced this amplicon. A PCR method amplifying a 400 bp fragment of the V. parahaemolyticus peptide chain release factor 1 could be useful in the specific and rapid detection of the species.