Design of a bacterial system using Escherichia coli to detect the mutagenic effect of some drugs

  • Abbas Mayar Hezam
Keywords: Keywords: Auxotroph's Escherichia coli, Ibuprofen, PCR, DNA sequencer


The current study aimed to use the auxotroph's tryptophan Escherichia coli as a test to detect Ibuprofen's mutagenic potential. the Ames test was used to detect reverse mutations in auxotroph's E. coli treated with Ibuprofen. The results showed a significant increase (P< 0.05) in reverse colonies of auxotroph's E. coli treated with 250 and 500 mg/mL of Ibuprofen compared to the negative control. The polymerase chain reaction and DNA Sequencing were used to detect the reverse mutations in the try gene of the auxotroph's E. coli. The results showed the presence of the try gene in all isolates treated with 250 and 500 mg/mL of Ibuprofen at a percentage of (100%). The try gene was sequenced using BLAST software and compared to the gene sequence of a standard isolate. The genetic variation analysis of the try gene isolates treated with Ibuprofen revealed that point mutations in the DNA of the try gene altered protein translation.


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How to Cite
Abbas Mayar Hezam. (2023). Design of a bacterial system using Escherichia coli to detect the mutagenic effect of some drugs. Al-Qadisiyah Journal of Pure Science, 27(1), 21-30.